​Revolutionizing Canine Leptospirosis Detection with PCR

The Limitations of Conventional Leptospirosis Testing

- **Bacterial Culture**: Requires specialized media and up to 3 months for results, making it impractical for urgent cases.

- **Serological Tests (e.g., MAT)**: Reliant on antibody detection, which can be masked by prior vaccination or delayed in acute infections. False positives/negatives are common, and paired sera samples are needed for confirmation.

- **Manual PCR**: While faster than culture, it demands extensive technical expertise, time-consuming sample preparation, and costly equipment—barriers for many veterinary clinics.

Introducing FLASHTEST: The Gold Standard in Leptospirosis Detection

FLASHTEST, developed by Wuxi OpuLen Technology Co., Ltd., is a cutting-edge real-time PCR assay designed specifically for veterinary use. This fully automated system combines DNA extraction, amplification, and detection into a single, user-friendly workflow, delivering results in **just 2 hours**. Here’s why FLASHTEST is transforming leptospirosis diagnostics:

1. Unrivaled Speed and Efficiency

- **Rapid Results**: Gone are the days of waiting weeks for answers. FLASHTEST provides actionable insights in 2 hours, enabling timely treatment decisions.

- **Automated Workflow**: Eliminates manual handling errors and reduces technician training time. Simply load the sample, and the system does the rest.

2. Exceptional Accuracy

- **Direct DNA Detection**: Identifies Leptospira spp. DNA directly in urine, blood, or tissue samples, even during the early leptospiremic phase when antibodies are undetectable.

- **Serovar-Specific Identification**: Through advanced sequencing, FLASHTEST can distinguish between pathogenic serovars like *L. canicola* (documented in 18.75% of Polish hunting dogs tested), critical for epidemiological tracking and vaccination strategies.

3. Clinical Validation

In a recent study published in *Życie Weterynaryjne*, FLASHTEST was used to screen 32 asymptomatic hunting dogs in Poland. Results revealed:

- **18.75% Positivity Rate**: Detected *L. canicola* in 6 dogs, highlighting the silent reservoir role of asymptomatic carriers.

- **Correlation with Thrombocytopenia**: All positive cases showed platelet abnormalities, underscoring the test’s ability to identify subclinical infections.

4. Field-Ready Design

- **Portable and Robust**: Ideal for mobile clinics, rural settings, or high-throughput laboratories.

- **Cost-Effective**: Reduces reagent waste and labor costs compared to traditional methods.

Why Choose FLASHTEST?

- **Human and Animal Health Protection**: Early detection prevents outbreaks and minimizes zoonotic risk.- **Vaccine Efficacy Monitoring**: Identifies vaccinated dogs shedding leptospires, ensuring herd immunity.- **Epidemiological Surveillance**: Provides critical data for targeted public health interventions.

Take the Lead in Animal Health

Don’t let leptospirosis go undetected. FLASHTEST empowers veterinarians to deliver precise, rapid diagnoses, safeguarding both pets and communities. Join leading clinics worldwide in adopting this innovative solution.