FLASHTEST PCR 1600 Real-Time qPCR Instrument

Content Summary of FLASHTEST PCR 1600 Real-Time qPCR Instrument

FLASHTEST PCR1600 delivers outstanding performance to speed up detection and improve accuracy. The flexible, easy-to-use system suits diverse settings and industries.

Faster detection:

• Results in 30 minutes

• 16 wells, 4-channel fluorescence detects up to 16 samples per run, covering 64 genes

Enhanced accuracy:

• 40 full amplification cycles

• Dual-zone heating enables precise temperature control

Simpler workflow:

• 10.1"" interactive LCD touchscreen

• One-touch start, no complex steps

Specification of FLASHTEST PCR 1600 Real-Time qPCR Instrument

Principle of Real-Time PCR Instrument/Machine

Real-Time PCR (Polymerase Chain Reaction), also known as quantitative PCR (qPCR), is a laboratory technique used to amplify and quantify DNA. The principle behind real-time PCR involves several key steps:

1. DNA Amplification:

The process starts with the amplification of specific DNA sequences using a polymerase enzyme. During PCR, DNA is denatured into single strands, annealed with specific primers, and then extended by DNA polymerase to form new strands. This cycle of denaturation, annealing, and extension is repeated multiple times to exponentially increase the number of DNA copies.

2. Fluorescence Detection:

Real-time PCR machines utilize fluorescent dyes or probes to monitor the amplification process in real time. Fluorescent dyes, such as SYBR Green, bind to double-stranded DNA and emit fluorescence when the DNA is amplified. Alternatively, specific fluorescent probes, like TaqMan probes, bind to the target DNA sequences and emit fluorescence upon cleavage during the extension phase.

3. Quantification:

The amount of fluorescence detected is proportional to the amount of DNA present. As the DNA amplification progresses, the fluorescence signal increases, allowing the real-time PCR machine to quantify the DNA in the sample. This quantification can be used to determine the initial amount of target DNA in the sample.

4. Data Analysis:

The data generated by the real-time PCR machine is analyzed to create amplification curves. These curves are used to calculate the quantification cycle (Cq) value, which reflects the number of cycles required for the fluorescence signal to exceed a predefined threshold. The Cq value is then used to estimate the initial concentration of the target DNA.