Mycoplasma Gallinis Wild Virus (MG)/Vaccine Strain (TS11) Nucleic Acid Test Kit (Lyophilized)

This two - item differential nucleic acid detection reagent mainly uses real - time polymerase chain reaction (qPCR) technology. Specific primers and probes are designed based on the genetic differences between the wild - type MG and the TS11 vaccine strain. The wild - type MG has unique genetic sequences in certain regions, such as the PvpA gene which is related to its virulence. The TS11 vaccine strain has specific genetic markers that distinguish it from the wild - type. During the qPCR process, the genomic DNA in the sample is amplified. Each set of primers and probes for the wild - type and the vaccine strain generates a distinct fluorescent signal. The real - time fluorescence detection system can accurately monitor and differentiate these signals, enabling rapid and accurate identification of whether the detected MG is a wild - type or the TS11 vaccine strain.

Clinical Applications

1. Poultry Farm Surveillance and Disease Source Identification：In poultry farms, regular screening with this reagent helps in early detection of MG. If respiratory symptoms occur in chickens, collecting tracheal swabs or nasal swabs and using this two - item differential detection reagent can quickly determine whether the pathogen is a wild - type MG or the TS11 vaccine strain. For example, in a large - scale broiler farm, if some chickens show respiratory problems, this test can clarify the source. If it is the wild - type MG, immediate isolation of the infected chickens, enhanced disinfection of the chicken coops, and appropriate antibiotic treatment can be carried out. If it is the TS11 vaccine strain, it may just be a normal reaction after vaccination, and only necessary monitoring is needed.

2. Vaccine Efficacy Monitoring：For farms that have implemented the TS11 vaccination program, this reagent is crucial for monitoring vaccine efficacy. Periodically testing samples from vaccinated chickens can help determine if the vaccine has successfully colonized and stimulated the immune response. If the TS11 vaccine strain is detected within a reasonable range after vaccination, it indicates that the vaccine is working well. However, if the wild - type MG is detected, it means there may be a problem with the biosecurity of the farm, and further measures are needed to prevent the spread of the wild - type virus.

3. Breeding Stock Management：When introducing new breeding chickens, screening them with this reagent can prevent the introduction of wild - type MG - infected birds into the breeding stock. This helps in preventing the vertical transmission of the wild - type virus to the next generation. If a breeding chicken is found to be positive for wild - type MG, it should be excluded from the breeding program. Regular testing of the breeding stock can also ensure the effectiveness of biosecurity measures in the breeding area.

4. Research on MG Epidemiology and Pathogenesis：In scientific research, this reagent allows researchers to study the prevalence and spread of wild - type MG and the distribution of the TS11 vaccine strain in different poultry populations. By analyzing a large number of samples, they can gain a better understanding of how the wild - type virus spreads, how the vaccine strain interacts with the host, and how to improve vaccine - related prevention and control strategies.